Rhodopsin

This volume provides readers with the latest information on the advances made in the field of rhodopsins. The chapters in this book cover topics such as new discoveries and developments; new ways to search for rhodopsins; methods to characterize the function and structure of rhodopsins on a molecular level; nano volume high throughput in meso crystallization, and fourth generation x-ray synchrotron sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Rhodopsin: Methods and Protocols is a valuable resource for any scientist and researcher interested in learning more about this developing field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Microbial Rhodopsins.- Molecular Biology of Microbial Rhodopsins.- Rhodopsin-Based Optogenetics: Basics and Applications.- Searching Metagenomes for New Rhodopsins.- E. coli Expression and Purification of Microbial and Viral Rhodopsins.- Crystallization of Microbial Rhodopsins.- Crystallographic Studies of Rhodopsins: Structure and Dynamics.- Time-Resolved UV-VIS Spectroscopy of Microbial Rhodopsins.- Solid-State NMR Spectroscopy on Microbial Rhodopsins.- FTIR and Raman Spectroscopy of Rhodopsins.- Single Cell Resolution Optogenetics via Expression of Soma-Targeted Rhodopsins.- Electrophysiological Characterization of Microbial Rhodopsins Transport Properties: Electrometric and pH Measurements using Planar Lipid Bilayer, Collodion Film and Fluorescent Probes Approaches.- Electrophysiological Characterization of Microbial Rhodopsins by Patch-Clamp Experiments.- Molecular Optimization of Rhodopsin-Based Tools for Neuroscience Applications.- Optogenetic Studies of Mitochondria.- In Vivo and In Vitro Characterization of Cyclase and Phosphodiesterase Rhodopsins.- Optogenetic Control of Human Stem Cell-Derived Neurons.