RNA Nanostructures

This volume details protocols for computer-assisted design and experimental characterization of RNA nanostructures. Chapters guide readers through RNA nanotechnology, design and characterization of RNA nanostructures, assessment of immunology of nanomaterials, biosensing and drug delivery, various biomedical applications, and delivery approaches for therapeutic RNA nanoparticles.Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge,RNA Nanostructures: Design, Characterization, and Applicationsaimsto address essential topics and concerns in the growing field of RNA nanotechnology.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Molecular Dynamics Simulations of RNA Motifs to Guide the Architectural Parameters and Design Principles of RNA Nanostructures.- Computer assisted design and characterization or RNA nanostructures.- Combining Experimental Restraints and RNA 3D Structure Prediction in RNA Nanotechnology.- Structural Characterization of Nucleic Acid Nanoparticles using SAXS and SAXS-driven MD.- Metalated Nucleic Acid Nanostructures.- Bioconjugation of Functionalized Oligodeoxynucleotides with Fluorescence Reporters for Nanoparticle Assembly.- Light-assisted drying for the thermal stabilization of nucleic acid nanoparticles and other biologics.- Preparation of Nucleic Acid Aptamer Functionalized Silver/Gold Nanoparticle Conjugates.- Thermodynamic Characterization of Nucleic Acid Nanoparticles Hybridization by UV Melting.- Structural Characterization of DNA Templated Silver Nanoclusters by Energy Dispersive Spectroscopy.- Small Volume microrheology to Evaluate Viscoelastic Properties of Nucleic Acid-based Hydrogels.- Characterization of RNA Nanoparticles and Their Dynamic Properties Using Atomic Force Microscopy.- Synthesis of Mesoporous Silica Nanoparticles for the Delivery of Nucleic Acid Nanostructures.- Assessment of intracellular compartmentalization of RNA nanostructures.- Discriminating immunorecognition pathways activated by RNA nanostructures.- Detection of nanoparticles' ability to stimulate toll-like receptors using HEK-Blue reporter cell lines.- Complexing PAMAM Dendrimers to Nucleic Acid Nanoparticles for their Intracellular Delivery.- Reverse transfection of functional RNA rings into cancer cells followed by in vitro irradiation.- RNA/DNA nanostructures designed for efficient regulation of blood coagulation.- Detection of multiplex NASBA RNA products using colorimetric split G quadruplex probes.- Synthesis of DNA-Templated Silver Nanoclusters and the Characterization of Their Optical Properties and Biological Activity.- Dynamic nanostructures for conditional activation and deactivation of biological pathways.- Anticoagulant activity of nucleic acid nanoparticles assessed by thrombin generation dynamics on a fully-automated system.