Selenoprotein Structure and Function: Volume 662

Informationen zum Autor Eranthie Weerapana is an Associate Professor of Chemistry at Boston College. She received her B.S. in Chemistry from Yale University, and her Ph.D. in Chemistry from MIT, where she worked with Professor Barbara Imperiali, investigating glycosyltransferases involved in N-linked glycosylation in the gram negative bacterium Campylobacter jejuni. She then performed postdoctoral studies at The Scripps Research Institute, La Jolla where she worked with Professor Benjamin F. Cravatt to develop chemical-proteomic methods to investigate reactive cysteines in complex proteomes. Her interdisciplinary research program focuses on applying mass-spectrometry methods to identify regulatory cysteine residues in the human proteome, and chemical biology approaches to develop covalent small-molecule modulators for cysteine-mediated protein activities.

Autorentext
Eranthie Weerapana is an Associate Professor of Chemistry at Boston College. She received her B.S. in Chemistry from Yale University, and her Ph.D. in Chemistry from MIT, where she worked with Professor Barbara Imperiali, investigating glycosyltransferases involved in N-linked glycosylation in the gram negative bacterium Campylobacter jejuni. She then performed postdoctoral studies at The Scripps Research Institute, La Jolla where she worked with Professor Benjamin F. Cravatt to develop chemical-proteomic methods to investigate reactive cysteines in complex proteomes. Her interdisciplinary research program focuses on applying mass-spectrometry methods to identify regulatory cysteine residues in the human proteome, and chemical biology approaches to develop covalent small-molecule modulators for cysteine-mediated protein activities.

Inhalt

1. Identification of Selenoprotein O substrates using a biotinylated ATP analog
   Anju Sreelatha
   2. Selenium-encoded isotopic signature targeted profiling
   Chu Wang
   3. Designing tRNASec variants for efficient selenocysteine incorporation using Sec-specific reporters
   Dieter Söll
   4. Preparation of selenoprotein S by chemical ligation
   Sharon Rozovsky
   5. Examining xCT-mediated selenium uptake and selenoprotein production capacity in cells
   Dohoon Kim
   6. SecMS analysis of selenoprotein with selenocysteine insertion sequence and beyond
   Yaoyang Zhang
   7. Selenocysteine substitutions in thiyl radical enzymes
   Brandon Greene
   8. Recombinant selenoprotein expression in E. coli based upon the redefinition of a UAG codon in an RF1-depleted host strain
   Elias S.J. Arnér
   9. Metabolic labeling with radioactive selenium in?zebrafish
   Paul Copeland
   10. Low pH isoTOP-ABPP to identify selenocysteines
   Daniel Bak
   11. Expression of selenoproteins via genetic code expansion in mammalian cells
   Jennifer Peeler
   12. Alpha-methyl selenocysteine as a tool for the study of selenoproteins
   Robert Hondal
   13. Selective selenol fluorescent probes: design, synthesis, structural determinants, and biological applications
   Jianguo Fang and Baoxin Zhang
   14. Diselenide-selenoester ligation
   Richard Payne
   15. Modeling selenocysteine-derived reactive intermediates utilizing a molecular cradle
   Kei Goto
   16. Methods for accurate and reproducible studies of pharmacological effects of selenium in cancer
   Mikael Björnstedt