Single Cell Diagnostics

The aim of Single Cell Diagnostics: Methods and Protocols is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells and moves through the techniques of standard fluorescence in situ hybridization and polymerase chain reaction (PCR) As the reader moves through the book, the scope and complexity of each technique gradually increases as real-time quantitative PCR, isothermal whole genome amplification, comparative genomic hybridization, real-time gene expression analysis and the production of RNA and cDNA libraries are covered. The book closes with the application of customized microarrays to the study of single cells.

Up-to-date, clinically applicable, validated protocols for single-cell diagnostics Whole genome amplification to create starting material from single cells Microarrays for analyzing multiple gene sequences simultaneously from a single cell Non-invasive assessment via polymerase chain reaction and in situ hybridization

Klappentext

This book applies modern molecular diagnostic techniques to the analysis of single cells, small numbers of cells, or cell extracts. Emphasis is placed on non-invasive analysis of single cell metabolites and the direct analysis of RNA and DNA from single cells, with a focus on polymerase chain reaction and fluorescence in situ hybridization. In particular, this handbook is essential for practitioners providing care for couples seeking treatment for infertility.

Inhalt
Noninvasive Metabolic Assessment of Single Cells.- Laser Capture Microdissection for Analysis of Single Cells.- Fluorescence In Situ Hybridization on Single Cells.- Single Cell Polymerase Chain Reaction for Preimplantation Genetic Diagnosis.- Real-Time Quantitative Polymerase Chain Reaction Measurement of Male Fetal DNA in Maternal Plasma.- Cell-Free Fetal DNA Plasma Extraction and Real-Time Polymerase Chain Reaction Quantification.- Linear-After-The-Exponential Polymerase Chain Reaction and Allied Technologies.- Efficient Isothermal Amplification of the Entire Genome from Single Cells.- Comparative Genomic Hybridization on Single Cells.- Generation of Amplified RNAs and cDNA Libraries from Single Mammalian Cells.- Use of Real-Time Polymerase Chain Reaction to Measure Gene Expression in Single Cells.- Gender Determination and Detection of Aneuploidy in Single Cells Using DNA Array-Based Comparative Genomic Hybridization.- Microarray Technology for Mutation Analysis of Low-Template DNA Samples.