Single Molecule Biology

Klappentext Single molecule techniques, including single molecule fluorescence, optical tweezers, and scanning probe microscopy, allow for the manipulation and measurement of single biological molecules within a live cell or in culture. These approaches, amongst the most exciting tools available in biology today, offer powerful new ways to elucidate biological function, both in terms of revealing mechanisms of action on a molecular level as well as tracking the behaviour of molecules in living cells. Zusammenfassung Describes such single molecule techniques (that allow for manipulation and measurement of single biological molecules within a live cell) as single molecule fluorescence! optical tweezers! and scanning probe microscopy. This book is organized by biological system or molecule. It discusses applications in molecular diagnostics and drug discovery. Inhaltsverzeichnis Introduction and Historical Perspective Motor Proteins: Linear Motors Actin and Tubulin-Based Motors DNA Motors Rotary Motors Single Molecule Enzymology Ribozymes Ribosomes Large Complexes Membrane Proteins Channels and Pores Cytoskeleton DNA Mechanics and Structure Single Molecules in Cells Hair Cells Structure of Single Protein Molecules Single Molecule Mechanical Properties Single Molecule Applications: Diagnostics Single Molecule Applications: Arrays Single Molecule Applications: Drug Discovery

Zusammenfassung
Describes such single molecule techniques (that allow for manipulation and measurement of single biological molecules within a live cell) as single molecule fluorescence, optical tweezers, and scanning probe microscopy. This book is organized by biological system or molecule. It discusses applications in molecular diagnostics and drug discovery.

Inhalt

Introduction and Historical Perspective
Motor Proteins: Linear Motors
Actin and Tubulin-Based Motors
DNA Motors
Rotary Motors
Single Molecule Enzymology
Ribozymes
Ribosomes
Large Complexes
Membrane Proteins
Channels and Pores
Cytoskeleton
DNA Mechanics and Structure
Single Molecules in Cells
Hair Cells
Structure of Single Protein Molecules
Single Molecule Mechanical Properties
Single Molecule Applications: Diagnostics
Single Molecule Applications: Arrays
Single Molecule Applications: Drug Discovery