Single Molecule Microscopy in Neurobiology

This volume looks at the methodology and techniques used by experts to study how certain molecules function in specific locations, and their temporal patterns. Chapters in this book cover topics such as in vivo single-molecule tracking of voltage-gated calcium channels with split-fluorescent proteins in CRISPR-engineering C. elegans; protein-protein interactions in membranes using single particle tracking; neuropathological diseases revealed by quantum-dot single particle tracking; SPoD-OnSPAN; and investigating molecular diffusion inside small neuronal compartments with two-photon fluorescence correlation spectroscopy. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Cutting-edge and comprehensive, Single Molecule Microscopy is a valuable resource for any researcher interested in learning more about this important field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Single Molecule Imaging of Intracellular Transport in Neurons and Non-Neuronal Cells: From Microscope Optics to Sample Preparations.- In Vivo Single-Molecule Tracking of Voltage-Gated Calcium Channels with Split-Fluorescent Proteins in CRISPR-Engineered C. elegans.- Nanocores and Liquid Droplets: Single-Molecule Microscopy of Neuronal Stress Granule Components.- Live-Cell Single-Molecule Imaging with Optogenetics Reveals Dynamics of an Activity-Dependent Transcription Factor.- Single-Molecule Imaging of Recycling Synaptic Vesicles in Live Neurons.- Approaching ProteinProtein Interactions in Membranes using Single Particle Tracking and Packing Coefficient Analysis.- Synaptic Function and Neuropathological Disease Revealed by Quantum-Dot Single Particle Tracking.- Multipolarization Darkfield Imaging of Single Endosomes in Microfluidic Neuronal Culture for Simultaneous Orientation and Displacement Tracking.- Practical Guidelines for Two-Color SMLM of Synaptic Proteins in Cultured Neurons.- Single-Molecule Localization Microscopy Propelled by Small Organic Fluorophores with Blinking Properties.- Highly-Biocompatible Super-Resolution Imaging: SPoD-OnSPAN.- Nanoscale Molecular Imaging of Presynaptic Active Zone Proteins in Cultured Hippocampal Neurons.- Three Dimensional Super-Resolution Imaging of the Cytoskeleton in Hippocampal Neurons using Selective Plane Illumination.- A Protocol for Single Molecule Translation Imaging in Xenopus Retinal Ganglion Cells.- Investigating Molecular Diffusion Inside Small Neuronal Compartments with Two-Photon Fluorescence Correlation Spectroscopy.