Site-Specific Protein Labeling

This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Chapters provide protocols for labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Site-Specific Protein Labeling: Methods and Protocols provides a comprehensive overview on the most relevant and established labeling methodologies, and helps researchers to choose the most appropriate labeling method for their biological question.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

How FlAsH got its Sparkle:Historical Recollections of the Biarsenical-tetracysteine Tag.- Site-Specific Protein Labeling in the Pharmaceutical Industry - Experiences from Novartis Drug Discovery.- Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins.- Considerations and Protocols for the Synthesis of Custom Protein Labeling Probes.- 2-Cyanobenzothiazole (CBT) Condensation for Site-Specific Labeling of Proteins at the Terminal Cysteine Residues.- Fluorescent Labeling for Patch Clamp Fluorometry (PCF) Measurements of Real-Time Protein Motion in Ion Channels.- Fluorescent Labeling Of SNAP-Tagged Proteins In Cells.- HaloTag Technology For Specific And Covalent Labeling Of Fusion Proteins.- Ligation Of Synthetic Peptides To Proteins Using Semisynthetic Protein Trans-Splicing.- Chemical-tag Labeling Of Proteins Using Fully Recombinant Split Inteins.- Phage Selection Assisted by Sfp Phosphopantetheinyl Transferase Catalyzed Site-Specific Protein Labeling.- Site-Specific Biotinylation Of Purified Proteins Using BirA.- Site-specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist.- BONCAT Metabolic Labeling, Click Chemistry, and Affinity Purification Of Newly Synthesized Proteomes.- Genetic Encoding Of Unnatural Amino Acids For Labeling Proteins.- Labeling Proteins By Affinity-Guided DMAP Chemistry.- Ligand-Directed Tosyl Chemistry For Selective Native Protein Labeling In Vitro, In Cells, and In Vivo.