Small Non-Coding RNAs

This volume contains state-of-the-art methods tackling all aspects of small non-coding RNAs biology. Small Non-Coding RNAs: Methods and Protocols guides readers through customized dedicated protocols and technologies that will be of valuable help to all those willing to contribute deciphering the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubles troubleshooting and avoiding known pitfalls.

Instructive and practical, Small Non-Coding RNAs: Methods and Protocols reaches out to biochemists, cellular and molecular biologists already working in the field of RNA biology and to those just starting to study small non-coding RNAs.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Autorentext
Mathieu Rederstorff, PhD Associate Professor Laboratoire IMoPA UMR 7365 CNRS/UL Equipe ARN, RNP - Maturation, Structure, Fonction - Université de Lorraine Biopôle/Campus Médecine Brabois 9 Avenue de la Forêt de Haye BP 184 54506 Vandoeuvre-lès-Nancy FRANCE Tel: +33 3 83 68 55 31 (office) +33 3 83 68 55 25 (lab) +33 6 49 12 67 11 (mobile) Fax: +33 3 83 68 55 09 (fax) mathieu.rederstorff@univ-lorraine.fr mathieu.rederstorff@maem.uhp-nancy.fr

Inhalt

Small Non-Coding RNAs: A Quick Look in the Rear-View Mirror.- Alcoholic Precipitation of Small Non-Coding RNAs.- Quantification and Quality Control of a Small Non-Coding RNA Preparation.- Impact of RNA Isolation Protocols on RNA Detection by Northern Blotting.- Improved Northern Blot Detection of Small RNAs Using EDC Crosslinking and DNA/LNA Probes.- Direct Cloning of Double Stranded RNAs.- Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation.- Fluorescence in situ Hybridization of Small Non-Coding RNAs.- RT-qPCR Based Quantification of Small Non-Coding RNAs.- Stem-Loop RT-PCR Based Quantification of Small Non-Coding RNAs.- miR-RACE: An Effective Approach to Accurately Determine the Sequence of Computationally Identified miRNAs.- Probing Small Non-Coding RNAs Structures.- cDNA Library Generation for the Analysis of Small RNAs by High Throughput Sequencing.- CLIP-seq to Discover Transcriptome-Wide Imprinting of RNA Binding Proteins in Living Cells.- Microarray Analysis of Small Non-Coding RNAs.- RLM-RACE, PPM-RACE and qRT-PCR, an Integrated Strategy to Accurately Validate miRNA Target Genes.- Dual Luciferase Gene Reporter Assays to Study miRNA Function.- Gene Expression Knockdown by Transfection of siRNAs into Mammalian Cells.- Efficient and Selective Knockdown of Small Non-Coding RNAs.- Cell-SELEX: In Vitro Selection of Synthetic Small Specific Ligands.- Small Non-Coding RNAs and Aptamers in Diagnostics and Therapeutics.