Staphylococcus Epidermidis

Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades. The chapters in Staphylococcus Epidermidis: Methods and Protocols are designed to give the new investigator a series of tools so they can ask novel and exciting questions related to the biology of this opportunistic pathogen, as many exciting and unexplored questions such as defining the interaction of S. epidermidis and other normal flora remain to be discovered. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Staphylococcus Epidermidis: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
S. epidermidis biology 1. S. epidermidis disease (Mark Rupp) 2. S. epidermidis pathogenesis/virulence factors (Michael Otto) 3. Isolation of the organism in the clinical microbiology laboratory (?) Molecular typing/population biology 4. Genome of S. epidermidis (Steve Gill) 5. Molecular epidemiology methodologies-PFGE, etc (not MLST) (Rich Goering) 6. Population biology methodologies-MLST (Ashley Robinson) Biofilm of S. epidermidis 7. Methods to visualize and analyze biofilm formation in Staphylococcus epidermidis. (Fey) 8. Polysaccharide Intercellular Adhesin and biofilm formation in Staphylococcus epidermidis. 9. Aap-mediated biofilm formation in S. epidermidis 10. Biofilm formation mediated by Embp in S. epidermidis Molecular Genetics and Tools 11. Transposons and IS elements of S. epidermidis 12. Allelic replacement methodologies in S. epidermidis. 13. Transposon mutagenesis protocols and tools 14. Transduction protocols in S. epidermidis Animal Models of Infection 15. Rat Catheter model (UNMC investigator) 16. Mouse flank model (Tammy Kielian) 17. Mouse orthopedic model (Carolyn, Mark, Kevin) 18. Central Nervous System S. epidermidis shut model of infection (Snowden)