Superantigens

This volume combines protocols that encompass the true variety of investigation done on superantigens in the fields of microbiology, immunology, molecular biology, biochemistry, and cellular biology, with a strong focus on disease models utilized to determine the role of superantigens in human disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Superantigens: Methods and Protocols contains a detailed and wide breadth of subject coverage that any scientist, clinician, or industry professional interested in this field will find valuable.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Animal Models Used to Study Superantigen-Mediated Diseases.- Identification, Purification, and Characterization of Staphylococcal Superantigens.- Strain Discrimination of Staphylococcus Aureus Using Superantigen Profiles.- Crystallization and Structure Determination of Superantigens and Immune Receptor Complexes.- Lipopolysaccharide-Induced Toxic Shock Syndrome in Rabbits.- Aortic Valve Damage for the Study of Left-Sided, Native Valve Infective Endocarditis in Rabbits.- Rabbit Model for Superantigen-Mediated Lethal Pulmonary Disease.- Nasopharyngeal Infection of Mice with Streptococcus Pyogenes and In Vivo Detection of Superantigen Activity.- Mini-Osmotic Pump Infusion Model to Investigate the Systemic Effects of Chronic Continuous Exposure to Staphylococcal Superantigen in Mice.- A Sub-Lethal Swine Model for Defining In Vivo Superantigen-Induced Responses Following Exposure to Staphylococcal Enterotoxin B.- Monkey Feeding Assay for Testing Emetic Activity of Staphylococcal Enterotoxin.- Use of an Ex-Vivo Porcine Mucosal Model to Study Superantigen Penetration.- Protocol for Examining Human Vaginal Epithelial Cell Signaling in Response to Staphylococcal Supe rantigens.- Organ Culture as a Model System for Studies on Enterotoxin Interactions with the Intestinal Epithelium.- Quantification of a Selective Expansion of T Cell Receptor V by Superantigen Using Real Time PCR.- Induction of Human Regulatory T Cells with Bacterial Superantigens.- Construction of Recombinant Single Chain Variable Fragment (ScFv) Antibody Against Superantigen for Immunodetection Using Antibody Phage Display Technology. <p