Synaptosomes

This volume describes techniques to make synaptosomes, and discusses numerous ways that synaptosomes are used to study and answer diverse questions about synapses.After examining the history that led to making synaptosomes, the chapters delved into its important as a tool to explore synaptic connections. Some of the topics covered in this book are: synaptosome bioenergetics and glutamate release; affinity proteomics for interactome and phosphoproteome screening in synaptosomes; isolation of highly purified and functionally active synaptic mitochondria; and profiling of microRNA and protein from purified synaptoneurosomes in a neurodegenerative disease model. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Cutting-edge and thorough, Synaptosomes is a valuable resource for any researcher interested in learning more about this developing field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Introduction to Synaptosome Methods and Applications.- Victor P. Whittaker: The Discovery of the Synaptosome and Its Implications.- Synaptosome Preparations: Which Procedure Should I Use?.- Protocol for a High-Throughput Semi-Automated Preparation for Filtered Synaptoneurosomes.- Subcellular Fractionation of Brain Tissue from Small Tissue Explants.- Preparation of P2 of Percoll-Purified Synaptosomes from Mammalian Brain Tissue.- Synaptosomal Bioenergetics and Glutamate Release.- Synaptosomes Bioenergetics and Calcium Handling: Aging Response.- Isolation of Highly Purified and Functionally Active Synaptic Mitochondria.- Affinity Proteomics for Interactome and Phosphoproteome Screening in Synaptosomes.- Manipulating Synapse-Enriched Biochemical Fractions to Detect a Purified Synaptic Protein's Effect on Neurotransmitter Release.- Prosomes: Supramolecular Structures at the Synaptosome Membrane Involved in Vesicle Docking, Fusion,and Neurotransmitter Release.- SMPL-Synaptic Membranes: Nanodisc-Mediated Synaptic Membrane Mimetics Expand the Toolkit for Drug Discovery and the Molecular Cell Biology of Synapses.- Profiling of MicroRNA and Protein from Purified Synaptoneurosomes in a Neurodegenerative Disease Model.- Use of Synaptoneurosome Samples to Study Development and Plasticity of Human Cortex.- The Use of Synaptosomes in Studying Autism Spectrum Disorder and Other Neurodevelopmental Disorders.- Flow Cytometric Evaluation of Crude Synaptosome Preparation as a Way to Study Synaptic Alteration in Neurodegenerative Diseases.