Techniques in Confocal Microscopy

Part of the "Reliable Lab Solutions" series, this title brings together chapters from volumes 302, 307 and 356 of "Methods in Enzymology". It documents many diverse uses for confocal microscopy in disciplines that broadly span biology. It describes techniques in a context that allows comparisons to other related methodologies.

Autorentext
P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public's awareness of diabetes. Conn's students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.

Klappentext

The topic of confocal microscopy and other important microscopic techniques is rapidly evolving, with growing value and interest in the area. As part of the Reliable Lab Solutions series, Techniques in Confocal Microscopy brings together chapters from volumes 302, 307 and 356 of Methods in Enzymology. It documents many diverse uses for confocal microscopy in disciplines that broadly span biology. The methods presented include shortcuts and conveniences not included in the initial publications and the techniques are described in a context that allows comparisons to other related methodologies. Methodologies are laid out in a manner that stresses their general applicability and reports their potential limitations. This volume provides a substantial and current overview of the extant methodology in the field and a view of its rapid development.

Inhalt

Part I. Practical Considerations and Equipment

1. Theoretical Basis of Confocal Microscopy
   Robert H. Webb
2. Practical Considerations in Acquiring Biological Signals from Confocal Microscope
   Hao He, Rose Chik Ying Ong, Kam Tai Chan, Ho Pui Ho, Siu Kai Kong
3. Equipment for Mass Storage and Processing of Data
   Guy Cox
4. Antifading Agents for Confocal Fluorescence Microscopy
   Miguel Berrios, Kimberly A. Conlon, and David E. Colflesh
5. Mounting Techniques for Confocal Microscopy
   Manabu Kagayama and Yasuyuki Sasano
6. Preparation of Whole Mounts and Thick Sections for Confocal Microscopy
   Hisashi Hashimoto, Hiroshi Ishikawa, and Moriaki Kusakabe
7. Use of Confocal Microscopy to Investigate Cell Structure and Function
   Ghassan Bkaily, Danielle Jacques

8. Combining Laser Scanning Confocal Microscopy and Electron Microscopy
   Xuejun Sun

   Part II. Functional Approaches
   9. Volume Measurements in Confocal Microscopy
   Carlos B. Mantilla, Y.S. Prakash and Gary C. Sieck

9. Quantitation of Phagocytosis by Confocal Microscopy
   George F. Babcock and Chad T Robinson MS
10. Receptor-Ligand Internalization
    Guido Orlandini, Nicoletta Ronda, Rita Gatti, Gian Carlo Gazzola, and Alberico Borghetti
11. Quantitative Imaging of Metabolism by Two-Photon Excitation Microscopy
    David W. Piston and Susan M. Knobel
12. Trafficking of the Androgen Receptor
    Virginie Georget, Béatrice Terouanne, Jean-Claude Nicolas, and Charles Sultan
13. Localization of Proteases and Peptide Receptors by Confocal Microscopy
    Kanti D. Bhoola, Celia J. Snyman and Carlos D. Figueroa
14. Multiphoton Excitation Microscopy, Confocal Microscopy, and Spectroscopy of Living Cells and Tissues: Functional Metabolic Imaging of Human Skin in Vivo
    Barry R. Masters, Peter T. C. So, Ki Hean Kim, Christof Buehler, and Enrico Gratton
15. Video-Rate, Scannng Slit Confocal Microscopy of Living Human Cornea in Vivo: Three-Dimensional Confocal Microscopy of the Eye
    Barry R. Masters and Matthias Böhnke
16. In Vivo Imaging of Mammalian Central Nervous System Neurons with the In Vivo Confocal Neuroimaging (ICON) Method
    Sylvia Prilloff, Petra Henrich-Noack, Ralf Engelmann, Bernhard A. Sabel

17. Identification of Viral Infection by Confocal Microscopy
    David N. Howell and Sara E. Miller
    19. Membrane Trafficking
    Sabine Kupzig, San San Lee, and George Banting

    Part III. Green Fluorescent Protein

18. Monitoring of Protein Secretion with Green Fluorescent Protein
    Christoph Kaether and Hans-Hermann Gerdes
19. Comparison of Enhanced Green Fluorescent Protein and Its Destabilized Form as Transcription Reporters
    Xiaoning Zhao, Tommy Duong, Chiao-Chian Huang, Steven R. Kain, and Xianqiang Li
20. Measuring Protein Degradation with Green Fluorescent Protein
    Stephen R. Cronin and Randolph Y. Hampton
21. Studying Nuclear Receptors with Green Fluorescent Protein Fusions
    Gordon L. Hager
22. Signaling, Desensitization, and Trafficking of G Protein-Coupled Receptors Revealed by Green Fluorescent Pro…