The Plant Plasma Membrane

The plasma membrane forms the living barrier between the cell and its surroundings. For this reason it has a wide range of important functions related to the regulation of the composition of the cell interior and to com munication with the cell exterior. The plasma membrane has therefore attracted a lot of research interest. Until the early 1970's it was only pos sible to study the plasma membrane in situ, its structure e. g. by electron microscopy and its function e. g. by uptake of radioactively labeled com pounds into the intact cell or tissue. The first isolation of plant protoplasts by enzymatic digestion of the cell wall in the early 1970's was an important step forward in that it provided direct access to the outer surface of the plasma membrane. More importantly, T. K. Hodges and R. J. Leonard in 1972 published the description of a method by which a fraction enriched in plasma membranes could be isolated from plant tissues using sucrose gradient centrifugation. As a result, the 1970's saw a leap forward in our understanding of the structurc and function of the plasma membrane. In 1981, S. Widell and C. Larsson published the first of a series of papers in which plasma membrane vesicles of high yield and purity were isolated from a wide range of plant tissues using aqueous polymer two-phase parti tioning.

Klappentext

This is the first book covering all aspects of the plant plasma membrane, thus presenting a comprehensive overview of the entire field. For everyone involved in plant cell research this book will serve equally well as a valid handbook. For each subject area an internationally recognized expert has been chosen to give his/her personal view of the present state of knowledge in the particular area including all relevant, newly developed techniques. To give an integrated picture of the plasma membrane these separate chapters have been rigorously edited by Larsson and Moller, themselves experts in the field of biochemistry of plasma membranes. This review discusses the results concerning molecular composition and organization of the plasma membrane, in particular the problem of plasma membrane markers, methods of membrane isolation and cytochemical approaches are discussed as well as topics such as redox systems, ATPases and transport in plasma membrane vesicles, patch clamp technique, signal transduction, coated pits, cellulose synthesis and plasma membrane/cytoskeleton interactions. The role of plasma membranes in the adaptation to cold and frost, during infection by pathogens and in symbiosis is addressed. A final chapter outlining molecular biological perspectives complements this overview.

Inhalt
1 An Introduction to the Plant Plasma Membrane - Its Molecular Composition and Organization.- 2 A Critical Evaluation of Markers Used in Plasma Membrane Purification.- 3 Plasma Membrane Isolation.- 4 Plasma Membrane Cytochemistry.- 5 Redox Processes in the Plasma Membrane.- 6 Plasma Membrane ATPase.- 7 Transport in Plasma Membrane Vesicles - Approaches and Perspectives.- 8 Electrophysiology of the Plasma Membrane of Higher Plant Cells: New Insights from Patch-Clamp Studies.- 9 Signal Sensing and Signal Transduction Across the Plasma Membrane.- 10 Coated Pits.- 11 Role of the Plasma Membrane in Cellulose Synthesis.- 12 The Plasma Membrane-Associated Cytoskeleton.- 13 Responses of the Plasma Membrane to Cold Acclimation and Freezing Stress.- 14 Role of the Plasma Membrane in Host-Pathogen Interactions.- 15 The Role of the Plant Plasma Membrane in Symbiosis.- 16 Molecular Biology of the Plasma Membrane Perspectives.