The Wilms' Tumor (WT1) Gene

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains keynotes and implementation advice from the experts Includes supplementary material: sn.pub/extras

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WT1 Mutation in Childhood Cancer.- WT1 and Renal Disease.- The Role of WT1 in Embryonic Development and Normal Organ Homeostasis.- Tools and Techniques for WT1-Based Lineage Tracing.- Biological Systems and Methods for Studying WT1 in the Epicardium.- Isolation and Colony Formation of Murine Bone and Bone Marrow Cells.- Isolation and Fluorescent Activated Cell Sorting of Murine WT1-Expressing Adipocyte Precursor Cells.- In Vivo Assays for Assessing the Role of the Wilms' Tumor Suppressor 1 (WT1) in Angiogenesis.- Multiphoton Microscopy for Visualizing Lipids in Tissue.- Function and Regulation of the Wilm's Tumor Suppressor 1 (WT1) Gene in Fish.- Immunofluorescence Staining of WT1 on Sections of Zebrafish Embryos and Larvae.- Fluorescence Activated Cell Sorting (FACS) Protocol for Podocyte Isolation in Adult Zebrafish.- In Vitro Transcription to Study WT1 Function.- Measuring Equilibrium Binding Constants for the WT1-DNAInteraction Using a Filter Binding Assay.- Identifying Direct Downstream Targets: WT1 ChIP-Seq Analysis.- WT1 Associated Protein-Protein Interaction Networks.- Methods to Identify and Validate WT1-RNA Interaction.- Bioinformatic Analysis of Next Generation Sequencing Data to Identify WT1 Associated Differential Gene and Isoform Expression.- Immunotherapy Targeting WT1: Designing a Protocol for WT1 Peptide-Based Cancer Vaccine.