The Zebrafish: Genetics, Genomics and Informatics: Volume 135

This volume on genetics, genomics, and informatics, will cover new technologies in forward and reverse genetics, transgenesis, the zebrafish genome and mapping technologies, informatics and comparative genomics, and Infrastructure. This volume of Methods in Cell Biology will prove valuable both to seasoned zebrafish investigators as well as to those who are newly adopting the zebrafish model as part of their research armamentarium.

Autorentext
Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.

Zusammenfassung
Focuses on Genetics, Genomics, and Informatics, covering various technologies in Forward and Reverse Genetics, Transgenesis, The Zebrafish Genome and Mapping Technologies, Informatics and Comparative Genomics, and Infrastructure.

Inhalt

Part I: Forward and Reverse Genetics

1. Gene Trap Mutagenesis for Generation of Conditional Mutations in Zebrafish - Wenbiao Chen

2. Advances in Tol2 Transposon Mutagenesis - Koichi Kawakami

3. OPEN Zinc Finger Nucleases - Keith Joung and Randy Peterson

4. Retroviral-Mediated Insertional Mutagenesis in Zebrafish - Adam Amsterdam, Nancy Hopkins, and Shawn Burgess

5. Genetic Screens for Maternal-Effects Mutations - Francisco Pelegri and Mary Mullins

6. High-Throughput Target-Selected Gene Inactivation in Zebrafish - Derek Stemple

7. Suppressor Screens in Haploids - Xiaoying Bai

8. Transposons - Stephen Ekker

9. Caged MOs - James Chen

   Part II: Transgenesis

10. Advanced Transgenesis with Cre Recombinase - Christian Mosimann

11. Transgenesis with phiC31 Recombinase - James Lister

12. Nuclear Transfer - Jose Cibelli and Dr. Siripattarapravat

    Part III: The Zebrafish Genome and Mapping Technologies

13. The Zebrafish Genome Project: Sequence Analysis and Annotation- Derek Stemple

14. Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC) Probe Panel Resource for Genomic Analyses in Zebrafish - Charles Lee and Amanda Smith

15. Automated Analysis of Conserved Syntenies for the Zebrafish Genome - John Postlethwait, Julian Catchen

16. The Zon Laboratory Guide to Positional Cloning in Zebrafish -Yi Zhou and Leonard Zon

    Part IV: Informatics and Comparative Genomics

17. Data Warehouse ZFIN - Monte Westerfield and Judy Sprague

18. Epigenetics: Methylation - Brad Cairns

19. Chromatin Structure - Leonard Zon and Yi Zhou

20. ChipSeq - Brad Cairns

21. Micro RNAs - Graham Lieshke and Luke Pase

22. Microarrays - Yi Zhou

23. Sequence-Based Transcriptional Profiling - Shawn Burgess

24. Epigenetic Regulation of Zebrafish Development - Elke Ober and co-author

    Part V: Infrastructure

25. Zebrafish Facilities for Small and Large Laboratories - Christian Lawrence

26. Genetic Backgrounds, Standard Lines, and their Husbandry - ZIRC, Zoltan