Theory and Strategy in Histochemistry

This book has been written to help scientists in biomedical research and routine service laboratories select and develop histochemical and cytochemical techniques. It does not give exhaustive methodological descriptions, but instead answers questions like "How can I apply histochemistry to my investigation?" "What are some typical problems?" "How should I interpret the results?"

Inhalt
1: General Considerations.- 1 The Scope of Histochemistry.- 2 The Structural and Chemical Basis for Histochemistry.- 3 Reagents.- 2: Review of Techniques According to Mechanism.- 4 General Theory for Tissue Staining.- 5 Blocking and Deblocking Reactions.- 6 Staining of Macromolecules on the Basis of Charge.- 7 Staining Involving Metal Complex Dyes.- 8 Staining Based on Reductants and Oxidants.- 9 Staining Involving Covalent Bonds.- 3: Tissue Processing.- 10 Tissue Processing I: Overview.- 11 Tissue Processing II: Freezing.- 12 Tissue Processing III: Fixation, General Aspects.- 13 Tissue Processing IV: Applied Fixation.- 14 Tissue Processing V: Embedding.- 15 Tissue Processing VI: Hard Tissues.- 16 Tissue Processing VII: Post Treatment.- 4: The Staining of Chemical Entities.- 17 Metals and Metal Salts.- 18 Pigments.- 19 Lipids.- 20 Nucleic Acids.- 21 Proteins.- 22 Carbohydrates.- 5: Enzyme Histochemistry.- 23 Enzyme Histochemistry I. General Considerations.- 24 Enzyme Histochemistry II. Hydrolases.- 25 Enzyme Histochemistry III. Oxidoreductases.- 6: Other Techniques.- 26 Immunohistochemistry.- 27 Ultrastructural Cytochemistry and Immunocytochemistry.- 28 Quantitation in Histochemistry.- 29 Autoradiography.- 30 Fluorescence Microscopic Methods in Histochemistry.- 7: An Introduction to Applied Histochemistry.- 31 Applied Histochemistry An Overview.- 32 Applied Immunohistochemistry.- Appendix A: Standardization of Staining Methods.- A.I General Considerations.- A.2 Examples of Staining Methods.- Appendix B: Quantitative Methods in Microscopy.- B.I Definitions.- B.2 Observations.- B.3 Stereology.- B.4 Special Stereological Tools.- B.5 Simple Counting Procedures.- B.6 Manipulating Digital Images.- B.7 Applications of Stereology in Pathology.- References.- Index ofConstituents.- Index of Dyes.- Index of Methods.