Thermal Effects and Temperature Profiles in Capillary Electrophoresis

Measuring Temperature without Joule heating effects in CE: The introductory chapter gives a detailed description of thermal effects in capillary electrophoresis. The following experimental chapters describe simple methods to find the mean temperature of electrolyte inside the capillary, which do not involve any external equipment. The current and voltage data collected from the instrument is used to find the conductance, G at various applied voltages. By plotting a graph of G vs the power per unit length, P/L, a straight line is obtained which is extrapolated to P/L = 0 to find the conductance free of Joule heating G(0). The increase in temperature of the electrolyte is found from the change in G. Studies in fused silica- and polymer- capillaries are presented. A quick technique for fitting conductance versus voltage data to find the temperature increases is also described. Electroosmotic mobility may be used to find the temperature at the inner capillary wall. Techniques for finding limiting electrophoretic mobilities and for determination of the surface heat transfer coefficient are also presented. If you are interested in temperature measurement in CE, this is book for you.

Autorentext

Christopher completed his PhD in 2007. He worked for 2 years as a post-doctoral fellow at the Australian Center for Research on Separation Science in ion chromatography. More recently he traveled to York University, Toronto, Ontario, Canada where he is researching Free Flow Electrophoresis and extending his work on temperature measurement in CE.