Tn3 Resolvase
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High Quality Content by WIKIPEDIA articles! Tn3 resolvase or is one out of three proteins that are encoded on the 4957 bp Tn3 transposon. Apart from Tn3 resolvase or tnpR, this transposon also carries a transposase or tnpA and a ?-lactamase Bla that confers resistance to ?-lactam antibiotics. Initially discovered as a repressor of transposase, resolvase also plays a role in facilitating Tn3 replication (Sherratt 1989). Tn3 replicates by intermolecular integrative transposition. This process is catalysed by the Tn3 transposase and it results in a fusion of the original host DNA with the target DNA molecule creating a ?cointegrate? along with the replication of the transposon. To separate the host and target molecules Tn3 resolvase executes site-specific recombination between the old and new copy of transposon at a speciffic site called res, which is present in each copy of the transposon. Res is 114 bp long and it consists of 3 sub-sites, namely sites I, II and III. Each of these sites is of different lengths (28, 34 and 25bp, respectively) and they are unevenly spaced with 22bp separating sites I and II and only 5bp between sites II and III.
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GarantieWeitere Informationen
- Allgemeine Informationen
- GTIN 09786130528003
- Editor Lambert M. Surhone, Miriam T. Timpledon, Susan F. Marseken
- EAN 9786130528003
- Format Fachbuch
- Titel Tn3 Resolvase
- Herausgeber Betascript Publishing
- Anzahl Seiten 120
- Genre Biologie
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