Tools and Trends in Bioanalytical Chemistry

This textbook covers the main tools and techniques used in bioanalysis, provides an overview of their principles, and offers several examples of their application and future trends in diagnosis.

Chapters from expert contributors explore the role of bioanalysis in different areas such as biochemistry, physiology, forensics, and clinical diagnosis, including topics from sampling/sample preparation, chemometrics in bioanalysis to the latest techniques used in the field. Particular attention is given to the recent advances in the application of mass spectrometry, NMR, electrochemical methods and separation techniques in bioanalysis. Readers will also find more about the application of microchip-based devices and analytical microarrays.

This textbook will appeal to graduate/advanced undergraduate students in Chemistry, Biology, Biochemistry, Pharmacy, and Chemical Engineering. It is also a useful resource for researchers and professionals working in the fields of biomedicine and veterinary sciences, with clear explanations and examples of how the different bioanalytical devices are applied for clinical diagnosis.

Covers the latest analytical techniques applied in bioanalysis Presents basic concepts and applications Offers several examples of application for clinical diagnosis

Autorentext
Lauro Tatsuo Kubota is a Full professor at the Chemistry Institute from the State University of Campinas (UNICAMP), Brazil, and a Research Fellow from the Brazilian National Council for Scientific and Technological Development (CNPq). He holds a Ph.D. in Chemistry from the University of Campinas (Brazil) and a Post-Doctorate in Analytical Chemistry from Lund University (Sweden) in 1997/98. Currently is the Coordinator of the National Institute on Science and Technology in Bioanalytics (INCTBio). He is a member of the São Paulo State Academy of Science (ACIESP) and the Brazilian Academy of Science (ABC) and became a Fellow of the Royal Society of Chemistry (RSC) in 2015. His main interest in researches focuses on Chemistry with emphasis on (Bio)Electroanalytical Chemistry for the development of sensors and biosensors employing novel materials, having published over 385 articles in specialized journals with a selective editorial policy and being author/co-author of 25 patents.

José Alberto Fracassi da Silva is an Associate Professor at the Chemistry Institute from the State University of Campinas (UNICAMP), Brazil, and holds a Ph.D. in Analytical Chemistry. He is also Associate Director at the Centre for Semiconductor Components and Nanotechnology (CCSNano) at UNICAMP and the Area Coordinator of the National Institute on Science and Technology in Bioanalytics (INCTBio). His main research interests are focused on bioanalytical applications involving oxygen and nitrogen reactive species, instrumentation and methods for capillary electrophoresis, and microfabrication strategies and materials for lab on a chip microsystems and integrated sensors. He has published 79 scientific papers, 5 book chapters and authored/co-authored 4 patents.

Marcelo Martins Sena is an Associate Professor at the Federal University of Minas Gerais, Belo Horizonte, Brazil, and Research Fellow from the Brazilian National Council for Scientific and Technological Development. He holds a Ph.D. in Chemistry, and his current work focuses on chemometrics in analytical chemistry, including the application of spectroscopic techniques (NIR, MIR, UV-vis, spectrofluorimetry) to the analysis of food, pharmaceuticals, agricultural products and forensic samples. He has also published +60 articles in international peer-reviewed journals.

Wendel Andrade Alves is an Associate Professor at the Federal University of ABC, Brazil, and Research Fellow from the Brazilian National Council for Scientific and Technological Development (CNPq). He holds a Ph.D. in Chemistry from the University of São Paulo, Brazil, and a Post-Doctorate in Physical Chemistry at the Electroactive Materials Laboratory from the same Institution. His research interests include supramolecular assembly of natural and synthetic polymers, self-assembly of peptides and biosensors.

Inhalt
What is bioanalytical chemistry? Scientific opportunities with immediate impact.- Role of bioanalytical chemistry in the 21st century.- Sampling and sample preparation in bioanalysis.- UV-VIS absorption and fluorescence in bioanalysis.- High-resolution techniques based on atomic and emission spectrometry applied to bioanalytical purposes.- Vibrational spectroscopy in bioanalysis.- Biological systems investigated by smal-angle X-ray scattering.- High resolution optical fluorescence microscopy for cell biology studies.- Nuclear magnetic resonance spectroscopy in analyses of biological samples.- SPR sensors: From configurations to bioanalytical applications.- Introduction to electroanalysis.- Amperometric detection for bioanalysis.- Potentiometric biosensors.- Electrochemical methods applied for bioanalysis. Differential pulse voltammetry and square wave voltammetry.- Impedimetric immunosensors for clinical practices: Focus on point-of-care diagnostics.- Organic electrochemical transistors in bioanalytical chemistry.- Quartz crystal microbalance in bioanalysis.- Scanning electrochemical microscopy (SECM): Fundamentals and applications.- Modified electrodes surface with inorganic oxides and conducting polymers.- The role of gas chromatography in bioanalysis.- Liquid chromatography in bioanalysis.- The role of capillary electromigration separation techniques in bioanalysis.- Introduction to bioanalytical mass spectrometry.- Microchip-based devices for bioanalytical applications.- Microarrays application in life sciences The beginning of the revolution.- Chemometrics in bioanalytical chemistry.- Recent advances and future trends in bioanalytical chemistry.