Transport and Receptor Proteins of Plant Membranes

This book presents the contributions of the main speakers to the 12th Long Ashton InternationalSymposium, heldat theUniversityofBristolfrom 17thto20thSeptember 1991. Manyofthe 160delegates who attended presented posters, the abstractsofwhich have been published separately and reflected thevigour, excitementand originalityofthepresentations. The identity and molecular structure of solute of membrane proteins are of interest in of themselves but may be seen as only a step towards broader questions about the regulation their activity and their integration into the lifeofcells and organisms. This symposium was held at a time when exciting progress was being made on the molecular biology of some transporters, ion channels and hormone receptors. The contributions reflect this progress in different degrees. Some problems are less tractable than others and the mixture of success stories and hopeful aspirations was a deliberate choice by the organisers. At the symposium those who were still confronted with great difficulties were listened to sympathetically and with constructive interest. Itis hoped that their papers will be read in the same way. During the symposium we discussed the natureofmany transport proteinsand receptors, as well as the existence ofadditional regulatory proteins such as protein kinases, G-proteins and calmodulin. Wecould say that upto twentyproteins wereconsidered inany detail. While this may sound like a considerable achievement, the scaleofourignorance can be illustrated byconsideringthatonatypicalsilver-stained, two-dimensionalpolyacrylamidegelofpurified plant plasma membrane polypeptides, between 150 and 200 spots are routinely shown; radiolabelled membranes frequently giveeven more, indicating how much membraneprotein there is, the function of which we have no conceptual framework.

Inhalt

1. Primary Active Transport Proteins.- A Plethora of Plant Plasmalemma Proton Pumps.- Studies on the Reaction Mechanism and Transport Function of P-type ATPases Associated with the Plant Plasma Membrane.- Vacuolar H+-translocating Inorganic Pyrophosphatase: Biochemistry and Molecular Biology.- Studies on the Higher Plant Calmodulin-Stimulated ATPase.- The Plasma Membrane Calcium Pump. Studies on Membrane Vesicles Isolated from Higher Plants.- 2. Secondary Ion and Metabolite Transporters.- Proton-Sugar Co-transporters in Plants.- Insights into the Structure of the Chloroplast Phosphate Translocator Protein.- Salt-Induced Changes in Ion Transport: Regulation of Primary Pumps and Secondary Transporters.- Uptake of Malate and Citrate into Plant Vacuoles.- 3. Channel Proteins.- Soybean Nodulin-26: A Channel Protein Conserved from Bacteria to Mammals.- The Application of Planar Lipid Bilayers to the Study of Plant Ion channels.- Inositol Phospholipid-Derived Signals in Plant Cells.- Putative L-type Calcium Channels in Plants: Biochemical Properties and Subcellular Localisation.- 4. Receptor Proteins.- Hormone Perception and Signal Transduction in Aleurone.- The Auxin Receptor: Structure and Distribution.- Membrane Receptors for Fusicoccin: A Molecular Study.- GTP-Binding Proteins in Higher Plant Cells.- 5. Protein Targeting and Assembly in Membranes.- Sequence Determinants for Protein Import into Chloroplasts and Thylakoid Membrane Protein Assembly.- Mechanism and Energetics of Protein Transport Across the Thylakoid Membrane.