Unconventional Protein Secretion

This volume presents relevant background information to understanding the molecular basis governing unconventional protein secretion (UPS), and in particular explores the latest techniques and protocols that have been successfully applied for the study of this topic. Detailed chapters include an overview of conventional and unconventional secretory pathways along with multidisciplinary approaches and methods used for UPS analysis in different organisms. Written in the highly successful Methods in Molecular Biology series format,chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Unconventional Protein Secretion: Methods and Protocols will beuseful for all interested in the secretory pathway field as well as applications in cell biology, cell development, biomedical research, and healthcare.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

ER to Golgi Dependent Protein Secretion: The Conventional Pathway.- Unconventional Protein Secretion in Animal Cells.- Unconventional Protein Secretion in Plants.- Chemical Secretory Pathway Modulation in Plant Protoplasts.- From Cytosol to the Apoplast: The Hygromycin Phosphotransferase (HYG R ) Model in Arabidopsis.- Following the Time-Course of Post-Pollination Events by Transmission Electron Microscopy (TEM): Buildup of Exosome-Like Structures with Compatible Pollinations.- Investigating Alternative Transport of Integral Plasma Membrane Proteins from the ER to the Golgi: Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR).- Quantification of a Non-Conventional Protein Secretion: The Low Molecular Weight FGF-2 Example.- Human Primary Keratinocytes as a Tool for the Analysis of Caspase-1-Dependent.- A Reporter System to Study Unconventional Secretion of Proteins Avoiding N -glycosylation in Ustilago maydis.- Stress-inducible Protein 1 (STI1): Extracellular Vesicles Analysis And Quantification.- Analysis of Yeast Extracellular Vesicles.- Exploring the Leishmania Hydrophilic Acylated Surface Protein B (HASPB) Export Pathway by Live Cell Imaging Methods.- Characterization of the Unconventional Secretion of the Ebola Matrix Protein VP40.- Role and Characterization of synuclein- Unconventional Protein Secretion in Cancer Cells.- Characterization of the Tumor Secretome from Tumor Interstitial Fluid (TIF).- Vacuum Infiltration-Centrifugation Method for Apoplastic Protein Extraction in Grapevin.- Isolation of Exosome-Like Vesicles from Plants by Ultracentrifugation on Sucrose/Deuterium Oxide (D 2 O) Density Cushions.