Vaccine Design

This volume provides a practical guide providing step-by-step protocol to explore vaccines for farm and companion animals, as well as for fish and insects. Divided into three volumes, Volume 2: Vaccines for Veterinary Diseases guides readers through veterinary vaccines, vaccines for poultry, vaccines for farm animals, and vaccines for veterinary parasites. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and practical, Vaccine Design: Methods and Protocols, Second Edition, Volume 2: Vaccines for Veterinary Diseases aims to be a useful practical guide to researchers to help further their study in this field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Challenges in Veterinary Vaccine Development.- Mammalian Cell Culture as a Platform for Veterinary Vaccines.- Avian Paramyxoviruses as Vectors for Vaccine Development.- Reverse Genetics and its Usage in the Development of Vaccine against Poultry Diseases.- Use of a Ferret Model to Test Efficacy and Immunogenicity of Live Attenuated Mycobacterium avium Subspecies Paratuberculosis Vaccines.- Recombinant Escherichia coli Cell lysates as a low-cost alternative for vaccines against veterinary clostridial diseases.- Clostridium spp. Toxins: A Practical Guide for Expression and Characterization in Escherichia coli.- Macrophage Stimulation as a Useful Approach for Immunoscreening of Potential Vaccine Candidates against Toxoplasma gondii and Neospora caninum Infections.- Fundamentals of Fish Vaccination.- Teleost Fish as an Experimental Model for Vaccine Development.- Development of Nano-conjugated DNA Vaccine against Edwardsiellosis Diseasein Fish.- Development and Evaluation of DNA Vaccine against Salmonid Alphavirus.- Novel Vaccine Development for Fish Culture Based on the Multiepitope Concept.- Testing Novel Inactivation Methods and Adjuvants for Vaccines against Streptococcus Agalactiae in Nile Tilapia Oreochromis niloticus.- Anti-tick Vaccines: Current Advances and Future Prospects.- Vaccines against Vector-Borne Diseases.- A quantum vaccinomics approach based on protein-protein interactions.- Screening for the 'Achilles Heel' of Hyalomma anatolicum Ticks by RNA Interference Technology and an Update on anti-Tick Vaccine Design.- Host Immunization with Recombinant Tick Antigen and Evaluation of Host Immune Response.- Fundamental Tick Vaccinomic Approach to Evade Host Autoimmune Reaction.