Visualization Techniques

This exploration of topics from immunohistochemistry for multiple neurochemicals covers a range of vital techniques from detecting expression levels of neurochemicals, to identifying polysynaptic pathways and recognizing functional changes in vivo.

Visualization of chemicals in tissues has seen incredible advances in the past several years. Visualization Techniques: From Immunohistochemistry to Magnetic Resonance Imaging provides practical advice from experts in the field as well as an excellent overview of some of the most important recent advances in visualization. This timely volume explores topics from immunohistochemistry for multiple neurochemicals, detecting expression levels of neurochemicals, following cellular processes and ionic movement, identifying polysynaptic pathways subserving physiological responses to identifying functional changes in vivo. Written for the popular Neuromethods series, this work includes the kind of detailed description and implementation advice that is crucial for getting optimal results in the lab.

Meticulous and concise, Visualization Techniques: From Immunohistochemistry to Magnetic Resonance Imaging will prove invaluable for scientists seeking to gain a greater understanding of the practical skills, strengths, and pitfalls that these wonderful and exciting visualization techniques provide.

Covers the top methods of visualizing neurochemistry Features step-by-step procedures to guide readers through key experiments Includes key tips and detailed implementation advice to ensure quality results

Inhalt

Multiple Immunohistochemical Labelling of Peripheral Neurons.- Combined In Situ Hybridization and Immunohistochemistry in Tat Brain Tissue Using Digoxigenin Labelled Riboprobes.- In Situ Hybridization within the CNS Tissue: Combining In Situ Hybridization with Immunofluorescence.- Visualizing GABA B Receptor Internalization and Intracellular Trafficking.- Using Total Internal Reflection Fluorescence Microscopy (TIRFM) to Visualize Insulin Action.- Live-Cell Quantification of Mitochondrial Functional Parameters.- Functional Imaging Using Two-Photon Microscopy in Living Tissue.- Calcium Imaging Techniques In Vitro to Explore the Role of Dendrites in Signaling Physiological Action Potential Patterns.- Juxtacellular Labeling in Combination with Other Histological Techniques to Determine Phenotype of Physiologically Identified Neurons.- Visualization of Activated Neurons Involved in Endocrine and Dietary Pathways Using GFP-Expressing Mice.- Use and Visualization of Neuroanatomical Viral Transneuronal Tracers.- Visualization of Thermal Changes in Freely Moving Animals.- Perfusion Magnetic Resonance Imaging Quantification in the Brain.