Wheat Biotechnology

This detailed volume provides background on recent new technology developments highlighting the potential of the genomic era in wheat breeding with invaluable instruction on the methodology, which is complemented by overview chapters on the status of new technology application in major wheat production countries. The topics, addressed by internationally renowned scientists active in the field, cover methods underpinning the latest developments in the field of wheat biotechnology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Wheat Biotechnology: Methods and Protocols serves as a vital resource for scientists working to breed future high-yielding wheat varieties to sustaina growing population in an increasingly unpredictable world.

Includes cutting-edge techniques for the study of biotechnological approaches to wheat production Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt

Enabling Molecular Technologies for Trait Improvement in Wheat.- What Will Be the Benefits of Biotech Wheat for European Agriculture?.- Overview of the Wheat Genetic Transformation and Breeding Status in China.- Wheat Improvement in India: Present and Future.- Overview of Methods for Assessing Salinity and Drought Tolerance of Transgenic Wheat Lines.- Allergenicity Assessment of Transgenic Wheat Lines In Silico.- Agribusiness Perspectives on Transgenic Wheat.- Agrobacterium-Mediated Transformation of Wheat Using Immature Embryos.- Biolistic Transformation of Wheat.- Wheat Genetic Transformation Using Mature Embryos as Explants.- Targeted Mutagenesis in Hexaploid Bread Wheat Using the TALEN and CRISPR/Cas Systems.- Design and Assembly of CRISPR/Cas9 Reagents for Gene Knock-Out, Targeted Insertion, and Replacement in Wheat.- Doubled Haploid Transgenic Wheat Lines by Microspore Transformation.- Doubled Haploid Laboratory Protocol for Wheat Using Wheat-Maize Wide Hybridization.- Real-Time PCR for the Detection of Precise Transgene Copy Number in Wheat.- Endogenous Reference Genes and Their Quantitative Real-Time PCR Assays for Genetically Modified Bread Wheat (Triticum aestivum L.) Detection.- Phenotypic Characterization of Transgenic Wheat Lines Against Fungal Pathogens Puccinia triticina and Fusarium graminearum.- Databases for Wheat Genomics and Crop Improvement.- High Density SNP Genotyping Array for Hexaploid Wheat and Its Relatives.