Xenotransplantation

In this book, experts study characterizations of xenogeneic interactions at the cellular and molecular levels and describe the use of relevant small-animal and pig-to-primate models. It also covers related ethical and legal considerations.

Despite many technological challenges faced by the xenotransplantation field, many major advances have been made in the last two decades. The field seeks to overcome the limitations and difficulties in organ procurement, which also apply to human cells and tissues, and facilitate the development of new therapies based on cell and engineered-tissue. Xenogeneic cells are simpler than solid organs and seem to pose less hurdles to attain long-term graft survival. In, Xenotransplantation: Methods and Protocols expert researchers study characterizations of xenogeneic interactions at the cellular and molecular levels and describe the use of relevant small-animal and pig-to-primate models. Related ethical and legal considerations are also covered. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Thorough and intuitive, Xenotransplantation: Methods and Protocols aids scientists in continuing to study xenotransplantation and its multiple aspects.

Covers many methods and subjects related to Xenotransplantation Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Xenotransplantation: An Overview of the Field.-Cloning and Expression Analyses of Pig Genes.-Basic Analyses of Proteins of Interest for Xenotransplantation.-Studies on Carbohydrate Xenoantigens.-Xenoantibodies and Complement Activity Determinations in Pig to Primate Xenotransplantation.-Studies on Coagulation Incompatibilities for Xenotransplantation.-Cellular Studies for in Vitro Modeling of Xenogeneic Immune Responses.-Production of Transgenic and Knock Out Pigs by Somatic Cell Nuclear Transfer.-Small Animal Models of Xenotransplantation.-Heart Xenotransplantation in Primate Models.-Xenogeneic Lung Transplantation Models.-Thymic Transplantation in Pig-to-nonhuman Primates for the Induction of Tolerance Across Xenogeneic Barriers.-Isolation of Porcine Pancreatic Islets for Xenotransplantation.-Pig Neural Cells Derived from Foetal Mesencephalon as Cell Source for Intracerebral Xenotransplantation.-Hepatocyte Xenotransplantation.-In Vitro Repair Model of Focal Articular Cartilage Defects in Humans.-Potential Zoonotic Infection of PERV in Xenotransplantation.-Ethical and Regulatory Issues for Clinical Trials in Xenotransplantation.-Some Ethical, Social and Legal Considerations of Xenotransplantation.